2024-03-29T14:40:25Z
https://researchonline.jcu.edu.au/cgi/oai2
oai:researchonline.jcu.edu.au:48140
2024-03-04T14:49:39Z
7374617475733D707562
74797065733D61727469636C65
Review of the recombinant human interferon gamma as an immunotherapeutic: Impacts of production platforms and glycosylation
Razaghi, Ali
Owens, Leigh
Heimann, Kirsten
Human interferon gamma is a cytokine belonging to a diverse group of interferons which have a crucial immunological function against mycobacteria and a wide variety of viral infections. To date, it has been approved for treatment of chronic granulomatous disease and malignant osteopetrosis, and its application as an immunotherapeutic agent against cancer is an increasing prospect. Recombinant human interferon gamma, as a lucrative biopharmaceutical, has been engineered in different expression systems including prokaryotic, protozoan, fungal (yeasts), plant, insect and mammalian cells. Human interferon gamma is commonly expressed in Escherichia coli, marketed as ACTIMMUNE®, however, the resulting product of the prokaryotic expression system is unglycosylated with a short half-life in the bloodstream; the purification process is tedious and makes the product costlier. Other expression systems also did not show satisfactory results in terms of yields, the biological activity of the protein or economic viability. Thus, the review aims to synthesise available information from previous studies on the production of human interferon gamma and its glycosylation patterns in different expression systems, to provide direction to future research in this field.
Elsevier
2016-12-20
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/48140/1/Razaghi%20et%20al_2016_Journal%20of%20Biotechnology_hIFN%20gamma%20expression_published.pdf
http://dx.doi.org/10.1016/j.jbiotec.2016.10.022
Razaghi, Ali, Owens, Leigh, and Heimann, Kirsten (2016) Review of the recombinant human interferon gamma as an immunotherapeutic: Impacts of production platforms and glycosylation. Journal of Biotechnology, 240. pp. 48-60.
https://researchonline.jcu.edu.au/48140/
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oai:researchonline.jcu.edu.au:50552
2024-02-29T14:33:42Z
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A preliminary investigation on the effect of extracorporeal shock wave therapy as a treatment for neurogenic heterotopic ossification following traumatic brain injury. Part I: effects on pain
Reznik, J.E.
Biros, E.
Lamont, A.C.
Sacher, Y.
Kibrik, O.
Milanese, S.
Gordon, S.
Galea, M.P.
Introduction: Neurogenic heterotopic ossification (NHO) is a complication of a neurological injury following traumatic brain injury (TBI) and may be present around major synovial joints. It is often accompanied by severe pain, which may lead to limitation in activities of daily living. Currently, a common intervention for NHO is surgery, which has been reported to carry many additional risks. This study was designed to assess the effect of extracorporeal shock wave therapy (ESWT) on pain in patients with TBI with chronic NHO.
Methods: A series of single-case studies (n = 11) was undertaken with patients who had TBI and chronic NHO at the hip or knee. Each patient received four applications of high-energy EWST delivered to the affected joint over 8 weeks. Two-weekly follow-up assessments were carried out, and final assessments were made 3 and 6 months post-intervention. Pain was measured using the Faces Rating Scale, and X-rays were taken at baseline and 6-months post-intervention to physiologically measure the size of the NHO.
Results: The application of high-energy ESWT was associated with significant overall reduction of pain in patients with TBI and NHO (Tau-0.412, 95% confidence interval -0.672 to -0.159, p = 0.002).
Conclusions: ESWT is a novel non-invasive intervention for reducing pain resulting from NHO in patients with TBI.
Informa Healthcare
2017
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/50552/1/50552%20Reznik%20et%20al%202017.pdf
http://dx.doi.org/10.1080/02699052.2017.1283059
Reznik, J.E., Biros, E., Lamont, A.C., Sacher, Y., Kibrik, O., Milanese, S., Gordon, S., and Galea, M.P. (2017) A preliminary investigation on the effect of extracorporeal shock wave therapy as a treatment for neurogenic heterotopic ossification following traumatic brain injury. Part I: effects on pain. Brain Injury, 31 (4). pp. 526-532.
https://researchonline.jcu.edu.au/50552/
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oai:researchonline.jcu.edu.au:50553
2024-02-29T14:33:43Z
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A preliminary investigation on the effect of extracorporeal shock wave therapy as a treatment for neurogenic heterotopic ossification following traumatic brain injury. Part II: effects on function
Reznik, J.E.
Biros, E.
Sacher, Y.
Kibrik, O.
Milanese, S.
Gordon, S.
Galea, M.P.
Introduction: Neurogenic heterotopic ossification (NHO) occurs as a complication of traumatic brain injury (TBI). Management of clinically significant NHO remains variable. Complications of mature NHO include limitation of mobility. The effect of the extracorporeal shock wave therapy (ESWT) on range of motion at hip and knee, and function in patients with TBI with chronic NHO was investigated.
Methods: A series of single-case studies applying ESWT to chronic NHO at the hip or knee of 11 patients with TBI were undertaken at a rehabilitation hospital. Participants received four applications of high-energy EWST delivered to the affected hip or knee over a period of 8 weeks. Two-weekly follow-up assessments were carried out; final assessments were made 3 and 6 months post-intervention. Range of motion (ROM) and Functional Reach (FR) or Modified Functional Reach (MFR) were measured.
Results: Application of high-energy ESWT was associated with significant improvement in ROM (flexion) of the NHO-affected knee (Tau = 0.833, 95% CI 0.391-1.276, p = 0.002) and significant improvement of FR (Overall Tau 0.486, 95% CI 0.141-0.832, p = 0.006); no significant improvement in hip ROM or MFR.
Conclusions: ESWT may improve mobility and balance of patients with TBI who have chronic NHO.
Informa Healthcare
2017
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/50553/1/50553%20Reznik%20et%20al%202017.pdf
http://doi.org/10.1080/02699052.2017.1283060
Reznik, J.E., Biros, E., Sacher, Y., Kibrik, O., Milanese, S., Gordon, S., and Galea, M.P. (2017) A preliminary investigation on the effect of extracorporeal shock wave therapy as a treatment for neurogenic heterotopic ossification following traumatic brain injury. Part II: effects on function. Brain Injury, 31 (4). pp. 533-541.
https://researchonline.jcu.edu.au/50553/
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oai:researchonline.jcu.edu.au:50863
2024-03-05T14:20:41Z
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Metallic biomaterials: current challenges and opportunities
Prasad, Karthika
Bazaka, Olha
Chua, Ming
Rochford, Madison
Fedrick, Liam
Spoor, Jordan
Symes, Richard
Tieppo, Marcus
Collins, Cameron
Cao, Alex
Markwell, David
Ostrikov, Kosya
Bazaka, Kateryna
Metallic biomaterials are engineered systems designed to provide internal support to biological tissues and they are being used largely in joint replacements, dental implants, orthopaedic fixations and stents. Higher biomaterial usage is associated with an increased incidence of implant-related complications due to poor implant integration, inflammation, mechanical instability, necrosis and infections, and associated prolonged patient care, pain and loss of function. In this review, we will briefly explore major representatives of metallic biomaterials along with the key existing and emerging strategies for surface and bulk modification used to improve biointegration, mechanical strength and flexibility of biometals, and discuss their compatibility with the concept of 3D printing.
MDPI Publishers
2017
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/50863/1/50863_Prasad%20et%20al_2017.pdf
https://doi.org/10.3390/ma10080884
Prasad, Karthika, Bazaka, Olha, Chua, Ming, Rochford, Madison, Fedrick, Liam, Spoor, Jordan, Symes, Richard, Tieppo, Marcus, Collins, Cameron, Cao, Alex, Markwell, David, Ostrikov, Kosya, and Bazaka, Kateryna (2017) Metallic biomaterials: current challenges and opportunities. Materials, 10 (8). 884.
https://researchonline.jcu.edu.au/50863/
open
oai:researchonline.jcu.edu.au:74720
2024-02-28T14:29:48Z
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74797065733D61727469636C65
Plasma polymers from oregano secondary metabolites: Antibacterial and biocompatible plant-based polymers
Romo-Rico, Jesus
Krishna, Smriti Murali
Golledge, Jonathan
Hayles, Andrew
Vasilev, Krasimir
Jacob, Mohan V.
Bacterial infection of chronic wounds is a major healthcare problem that affects the quality of life of millions of patients worldwide and leads to a substantial healthcare cost burden. This project focused on the manufacture of a potential wound healing agent. Plasma polymers from oregano secondary metabolites (PP-OSMs) were fabricated by radiofrequency plasma-enhanced chemical vapor deposition (RF-PECVD) in continuous and pulse plasma modes at room temperature. The surface, biocompatibility, and antibacterial properties of the PP-OSMs were investigated. Polymers fabricated by RF-PECVD retained the functional groups of OSMs, promoted human dermal fibroblast adhesion, inhibited Staphylococcus aureus attachment, and eliminated Pseudomonas aeruginosa. The PP-OSM coatings are potential candidates for use in medical applications where cell biocompatibility and antibacterial properties are required.
Wiley
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/74720/1/74720.pdf
https://doi.org/10.1002/ppap.202100220
Romo-Rico, Jesus, Krishna, Smriti Murali, Golledge, Jonathan, Hayles, Andrew, Vasilev, Krasimir, and Jacob, Mohan V. (2022) Plasma polymers from oregano secondary metabolites: Antibacterial and biocompatible plant-based polymers. Plasma Processes and Polymers, 19 (7). 2100220.
https://researchonline.jcu.edu.au/74720/
open
oai:researchonline.jcu.edu.au:76016
2024-02-29T14:45:51Z
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Potential of plant secondary metabolite-based polymers to enhance wound healing
Romo-Rico, Jesus
Krishna, Smriti Murali
Bazaka, Kateryna
Golledge, Jonathan
Jacob, Mohan V.
There is a global epidemic of non-healing wounds. Chronic inflammation, overexpression of pro-inflammatory cytokines, oxidative stress and bacterial infection are implicated in delayed wound healing. Natural extracts are a rich source of bioactive molecules called plant secondary metabolites (PSMs) that include terpenes and phenols. These molecules may facilitate wound healing through their antioxidant, anti-inflammatory, and antibacterial activity. After briefly outlining the process of wound healing and how it is compromised in chronic wounds, this review focuses on investigating how PSMs-based polymers may improve wound healing. Best methods for incorporating PSMs into wound dressings are reviewed and critically compared. The exiting body of literature strongly suggests that PSMs-based polymers incorporated into wound dressings could have clinical value in aiding wound healing.
Statement of significance: Chronic wounds develop by the persistence of inflammation, oxidative stress and infection. Chronic wounds affect the worldwide population, by reducing quality of life of patients with significant cost to healthcare systems. To help chronic wounds to heal and overcome this burden, materials with anti-inflammatory, antioxidant and antibacterial properties are required. Plant secondary metabolites (PSMs) are volatile materials that have all these properties. PSMs-based polymers can be fabricated by polymerization techniques. The present review provides an overview of the state-of-the-art of the wound healing mechanisms of PSMs. Current developments in the field of PSMs-based polymers are reviewed and their potential use as wound dressings is also covered. (c) 2022 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Elsevier
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/76016/1/76016.pdf
https://doi.org/10.1016/j.actbio.2022.05.043
Romo-Rico, Jesus, Krishna, Smriti Murali, Bazaka, Kateryna, Golledge, Jonathan, and Jacob, Mohan V. (2022) Potential of plant secondary metabolite-based polymers to enhance wound healing. Acta Biomaterialia, 147. pp. 34-49.
https://researchonline.jcu.edu.au/76016/
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oai:researchonline.jcu.edu.au:76397
2024-02-28T15:09:05Z
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A soft Tus-Ter interaction is hiding a fail-safe lock in the replication fork trap of Dickeya paradisiaca
Toft, Casey J.
Sorenson, Alanna E.
Schaeffer, Patrick M.
A variety of replication fork traps have recently been characterised in Enterobacterales, unveiling two different types of architecture. Of these, the degenerate type II fork traps are commonly found in Enterobacteriaceae such as Escherichia coli. The newly characterised type I fork traps are found almost exclusively outside Enterobacteriaceae within Enterobacterales and include several archetypes of possible ancestral architectures. Dickeya paradisiaca harbours a somewhat degenerate type I fork trap with a unique Ter1 adjacent to tus gene on one side of the circular chromosome and three putative Ter2–4 sites on the other side of the fork trap. The two innermost Ter1 and Ter2 sites are only separated by 18 kb, which is the shortest distance between two innermost Ter sites of any chromosomal fork trap identified so far. Of note, the dif site is located between these two sites, coinciding with a sharp GC-skew flip. Here we examined and compared the binding modalities of E. coli and D. paradisiaca Tus proteins for these Ter sites. Surprisingly, while Ter1–3 were functional, no significant Tus binding was observed for Ter4 even in low salt conditions, which is in stark contrast with the significant non-specific protein-DNA interactions that occur with E. coli Tus. Even more surprising was the finding that D. paradisiaca Tus has a relatively moderate binding affinity to double-stranded Ter while retaining an extremely high affinity to Ter-lock sequences. Our data revealed major differences in the salt resistance and stability between the D. paradisiaca and E. coli Tus protein complexes, suggesting that while Tus protein evolution can be quite flexible regarding the initial Ter binding step, it requires a highly stringent purifying selection for its final locked complex formation.
Elsevier
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/76397/1/76397.pdf
https://doi.org/10.1016/j.micres.2022.127147
Toft, Casey J., Sorenson, Alanna E., and Schaeffer, Patrick M. (2022) A soft Tus-Ter interaction is hiding a fail-safe lock in the replication fork trap of Dickeya paradisiaca. Microbiological Research, 263. 127147.
https://researchonline.jcu.edu.au/76397/
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oai:researchonline.jcu.edu.au:76989
2024-03-02T15:12:56Z
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Antimicrobial adhesive films by plasma-enabled polymerisation of m-cresol
Hartl, Hugo
Li, Wenshao
Michl, Thomas Danny
Anangi, Raveendra
Speight, Robert
Vasilev, Krasimir
Ostrikov, Kostya Ken
MacLeod, Jennifer
This work reveals a versatile new method to produce films with antimicrobial properties that can also bond materials together with robust tensile adhesive strength. Specifically, we demonstrate the formation of coatings by using a dielectric barrier discharge (DBD) plasma to convert a liquid small-molecule precursor, m-cresol, to a solid film via plasma-assisted on-surface polymerisation. The films are quite appealing from a sustainability perspective: they are produced using a low-energy process and from a molecule produced in abundance as a by-product of coal tar processing. This process consumes only 1.5 Wh of electricity to create a 1 cm2 film, which is much lower than other methods commonly used for film deposition, such as chemical vapour deposition (CVD). Plasma treatments were performed in plain air without the need for any carrier or precursor gas, with a variety of exposure durations. By varying the plasma parameters, it is possible to modify both the adhesive property of the film, which is at a maximum at a 1 min plasma exposure, and the antimicrobial property of the film against Escherichia coli, which is at a maximum at a 30 s exposure.
Nature Publishing Group
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/76989/1/76989.pdf
https://doi.org/10.1038/s41598-022-11400-8
Hartl, Hugo, Li, Wenshao, Michl, Thomas Danny, Anangi, Raveendra, Speight, Robert, Vasilev, Krasimir, Ostrikov, Kostya Ken, and MacLeod, Jennifer (2022) Antimicrobial adhesive films by plasma-enabled polymerisation of m-cresol. Scientific Reports, 12. 7560.
https://researchonline.jcu.edu.au/76989/
open
oai:researchonline.jcu.edu.au:76993
2024-03-02T15:12:59Z
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Biocompatible Polycationic Silver Nanocluster-Impregnated PLGA Nanocomposites with Potent Antimicrobial Activity
Uroro, Evelyn Osehontue
Bright, Richard
Dabare, Panthihage Ruvini L.
Bera, Debkumar
Quek, Jing Yang
Goswami, Nirmal
Vasilev, Krasimir
Ultrasmall cationic silver nanoparticles (AgNPs) have recently emerged as highly potent antimicrobial agents for the treatment of multidrug-resistant bacteria and their biofilms. However, the clinical application of these cationic AgNPs is hampered by their poor stability and high reactivity in solution, leading to uncontrolled release of toxic silver ions. An ideal platform featuring broad-spectrum antibacterial activity and high biocompatibility that prevents overexposure to silver ions, is therefore highly desirable. Herein, we explored a biocompatible and biodegradable polymer, poly(lactic-co-glycolic) acid (PLGA) as an effective carrier for the recently discovered polycationic silver nanoclusters (pAgNCs). These pAgNCs impregnated PLGA nanocomposites (pAgNCs@PLGA) were developed by water-in-oil-in-water (W1/O/W2) emulsion method and characterized by various analytical techniques. Our experimental results reveal that pAgNCs@PLGA had spherical morphology with an average diameter of ∼188 nm and consists of multiple ultrasmall (∼2 nm) pAgNCs at the polymeric core. The minimum inhibitory concentration of pAgNCs for Staphylococcus aureus and Pseudomonas aeruginosa were found to be 6.9 μg/mL. After impregnation within PLGA, the antimicrobial efficacy of our pAgNCs against Staphylococcus aureus and Pseudomonas aeruginosa remained consistent, while the nanocomposites were biocompatible at the minimum inhibitory concentration (MIC) against both bacteria. The pAgNCs@PLGA nanocomposite developed in this work may present a path forward to bring these highly potent pAgNCs into medical practice.
Wiley
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/76993/1/76993.pdf
https://doi.org/10.1002/cnma.202200349
Uroro, Evelyn Osehontue, Bright, Richard, Dabare, Panthihage Ruvini L., Bera, Debkumar, Quek, Jing Yang, Goswami, Nirmal, and Vasilev, Krasimir (2022) Biocompatible Polycationic Silver Nanocluster-Impregnated PLGA Nanocomposites with Potent Antimicrobial Activity. ChemNanoMat, 8 (11). e202200349.
https://researchonline.jcu.edu.au/76993/
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oai:researchonline.jcu.edu.au:76994
2024-03-02T15:13:13Z
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Plasma polymerization for biomedical applications: A review
Coad, Bryan R.
Favia, Pietro
Vasilev, Krasimir
Griesser, Hans J.
Plasma polymers have long been of interest as thin film coatings on biomedical devices and products, to generate desirable surface properties for favorable bio-interfacial interactions. Plasma polymers have also been used as platforms for the covalent immobilization of bioactive molecules. More recently, additional aspects have been investigated, such as selective prevention of adhesion of microbial pathogens, either via plasma polymers per se or including antimicrobial drugs. Plasma polymers have also been investigated for the release of silver ions and small organic molecules. Complementing low-pressure plasma approaches, processes at atmospheric pressure have attracted interest recently, including for nano/biocomposite coatings. This contribution reviews the use of plasma polymers for intended biomedical applications, with a focus on more recent topic areas.
Wiley
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/76994/1/76994.pdf
https://doi.org/10.1002/ppap.202200121
Coad, Bryan R., Favia, Pietro, Vasilev, Krasimir, and Griesser, Hans J. (2022) Plasma polymerization for biomedical applications: A review. Plasma Processes and Polymers, 19 (11). 2200121.
https://researchonline.jcu.edu.au/76994/
open
oai:researchonline.jcu.edu.au:77001
2024-03-02T15:13:40Z
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Surfaces Containing Sharp Nanostructures Enhance Antibiotic Efficacy
Bright, Richard
Hayles, Andrew
Wood, Jonathan
Palms, Dennis
Brown, Toby
Barker, Dan
Vasilev, Krasimir
The ever-increasing rate of medical device implantations is met by a proportionately high burden of implant-associated infections. To mitigate this threat, much research has been directed toward the development of antibacterial surface modifications by various means. One recent approach involves surfaces containing sharp nanostructures capable of killing bacteria upon contact. Herein, we report that the mechanical interaction between Staphylococcus aureus and such surface nanostructures leads to a sensitization of the pathogen to the glycopeptide antibiotic vancomycin. We demonstrate that this is due to cell wall damage and impeded bacterial defenses against reactive oxygen species. The results of this study promise to be impactful in the clinic, as a combination of nanostructured antibacterial surfaces and antibiotics commonly used in hospitals may improve antimicrobial therapy strategies, helping clinicians to prevent and treat implant-associated infections using reduced antibiotic concentrations instead of relying on invasive revision surgeries with often poor outcomes.
American Chemical Society
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/77001/1/77001.pdf
https://doi.org/10.1021/acs.nanolett.2c02182
Bright, Richard, Hayles, Andrew, Wood, Jonathan, Palms, Dennis, Brown, Toby, Barker, Dan, and Vasilev, Krasimir (2022) Surfaces Containing Sharp Nanostructures Enhance Antibiotic Efficacy. Nano Letters, 22 (16). pp. 6724-6731.
https://researchonline.jcu.edu.au/77001/
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oai:researchonline.jcu.edu.au:77006
2024-03-02T15:14:17Z
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Bio-Inspired Nanostructured Ti-6Al-4V Alloy: The Role of Two Alkaline Etchants and the Hydrothermal Processing Duration on Antibacterial Activity
Bright, Richard
Hayles, Andrew
Wood, Jonathan
Ninan, Neethu
Palms, Dennis
Visalakshan, Rahul M.
Burzava, Anouck
Brown, Toby
Barker, Dan
Vasilev, Krasimir
Inspired by observations that the natural topography observed on cicada and dragonfly wings may be lethal to bacteria, researchers have sought to reproduce these nanostructures on biomaterials with the goal of reducing implant-associated infections. Titanium and its alloys are widely employed biomaterials with excellent properties but are susceptible to bacterial colonisation. Hydrothermal etching is a simple, cost-effective procedure which fabricates nanoscale protrusions of various dimensions upon titanium, depending on the etching parameters used. We investigated the role of etching time and the choice of cation (sodium and potassium) in the alkaline heat treatment on the topographical, physical, and bactericidal properties of the resulting modified titanium surfaces. Optimal etching times were 4 h for sodium hydroxide (NaOH) and 5 h for potassium hydroxide (KOH). NaOH etching for 4 h produced dense, but somewhat ordered, surface nanofeatures with 75 nanospikes per µm2. In comparison, KOH etching for 5 h resulted sparser but nonetheless disordered surface morphology with only 8 spikes per µm2. The NaOH surface was more effective at eliminating Gram-negative pathogens, while the KOH surface was more effective against the Gram-positive strains. These findings may guide further research and development of bactericidal titanium surfaces which are optimised for the predominant pathogens associated with the intended application.
MDPI
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/77006/1/77006.pdf
https://doi.org/10.3390/nano12071140
Bright, Richard, Hayles, Andrew, Wood, Jonathan, Ninan, Neethu, Palms, Dennis, Visalakshan, Rahul M., Burzava, Anouck, Brown, Toby, Barker, Dan, and Vasilev, Krasimir (2022) Bio-Inspired Nanostructured Ti-6Al-4V Alloy: The Role of Two Alkaline Etchants and the Hydrothermal Processing Duration on Antibacterial Activity. Nanomaterials, 12. 1140.
https://researchonline.jcu.edu.au/77006/
open
oai:researchonline.jcu.edu.au:77009
2024-03-02T15:14:41Z
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Interactions between Liquid Metal Droplets and Bacterial, Fungal, and Mammalian Cells
Cheeseman, Samuel
Elbourne, Aaron
Gangadoo, Sheeana
Shaw, Z.L.
Bryant, Saffron J.
Syed, Nitu
Dickey, Michael D.
Higgins, Michael J.
Vasilev, Krasimir
McConville, Chris F.
Christofferson, Andrew J.
Crawford, Russell J.
Daeneke, Torben
Chapman, James
Truong, Vi Khanh
Liquid metals (LMs) have emerged as novel materials for biomedical applications. Here, the interactions taking place between cells and LMs are reported, presenting a unique opportunity to explore and understand the LM-biological interface. Several high-resolution imaging techniques are used to characterize the interaction between droplets of gallium LM and bacterial, fungal, and mammalian cells. Adhesive interactions between cells and LM droplets are observed, causing deformation of the LM droplet surface, resulting in surface wrinkling and in some cases, breakage of the native oxide layer present on the LM droplet surface. In many instances, the cell wall deforms to intimately contact the LM droplets. Single-cell force spectroscopy is performed to quantify the adhesion forces between cells and LM and characterize the nature of the adhesion. It is proposed that the flexible nature of the cell enables multiple adhesion sites with the LM droplets, imparting tensile forces on the LM droplet surface, which results in surface wrinkling on the LM droplets due to their liquid nature. Molecular dynamics simulations also suggest that flexible biomolecules on the cell surface can disrupt the Ga2O3 layer formed at the LM droplet surface. This study reveals a unique biointerfacial interaction and provides insights into the mechanisms involved.
Wiley
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/77009/1/77009.pdf
https://doi.org/10.1002/admi.202102113
Cheeseman, Samuel, Elbourne, Aaron, Gangadoo, Sheeana, Shaw, Z.L., Bryant, Saffron J., Syed, Nitu, Dickey, Michael D., Higgins, Michael J., Vasilev, Krasimir, McConville, Chris F., Christofferson, Andrew J., Crawford, Russell J., Daeneke, Torben, Chapman, James, and Truong, Vi Khanh (2022) Interactions between Liquid Metal Droplets and Bacterial, Fungal, and Mammalian Cells. Advanced Materials Interfaces, 9 (7). 2102113.
https://researchonline.jcu.edu.au/77009/
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oai:researchonline.jcu.edu.au:77012
2024-03-02T15:15:04Z
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The introduction of nanotopography suppresses bacterial adhesion and enhances osteoinductive capacity of plasma deposited polyoxazoline surface
Liu, Xujie
Yuan, Jiongpeng
Zhang, Jian
Visalakshan, Rahul Madathiparambil
Wang, Wenxia
Xiang, Yongxiao
He, Yan
Feng, Qingling
Vasilev, Krasimir
The plasma deposited polyoxazoline (PPOx) has been emerging in biomedical applications, especially for the surface modification of bone tissue engineering scaffold and/or bone implants. Herein, PPOx surfaces were generated by plasma polymerization with the introduction of surface nanotopography gradient, achieved by immobilization of different density of 16 nm gold nanoparticles. The introduction of surface nanotopography suppressed the adhesion of S. aureus on PPOx surface. Moreover, the introduction of surface nanotopography enhanced the initial attachment and spreading of hMSCs, as well as promoted the osteogenic differentiation of hMSCs. RhoA/ROCK signaling pathway may be involved in the enhancement of osteoinductive capacity of PPOx surface by nanotopography.
Elsevier
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/77012/1/77012.pdf
https://doi.org/10.1016/j.matlet.2021.131452
Liu, Xujie, Yuan, Jiongpeng, Zhang, Jian, Visalakshan, Rahul Madathiparambil, Wang, Wenxia, Xiang, Yongxiao, He, Yan, Feng, Qingling, and Vasilev, Krasimir (2022) The introduction of nanotopography suppresses bacterial adhesion and enhances osteoinductive capacity of plasma deposited polyoxazoline surface. Materials Letters, 309. 131452.
https://researchonline.jcu.edu.au/77012/
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oai:researchonline.jcu.edu.au:77014
2024-03-02T15:15:09Z
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A practical guide to promote informatics-driven efficient biotopographic material development
Guo, Yuanlong
Mi, Jiaomei
Ye, Chen
Ao, Yong
Shi, Mengru
Shan, Zhengjie
Li, Bingzhi
Chen, Zetao
Chen, Zhuofan
Vasilev, Krasimir
Xiao, Yin
Micro/nano topographic structures have shown great utility in many biomedical areas including cell therapies, tissue engineering, and implantable devices. Computer-assisted informatics methods hold great promise for the design of topographic structures with targeted properties for a specific medical application. To benefit from these methods, researchers and engineers require a highly reusable “one structural parameter – one set of cell responses” database. However, existing confounding factors in topographic cell culture devices seriously impede the acquisition of this kind of data. Through carefully dissecting the confounding factors and their possible reasons for emergence, we developed corresponding guideline requirements for topographic cell culture device development to remove or control the influence of such factors. Based on these requirements, we then suggested potential strategies to meet them. In this work, we also experimentally demonstrated a topographic cell culture device with controlled confounding factors based on these guideline requirements and corresponding strategies. A “guideline for the development of topographic cell culture devices” was summarized to instruct researchers to develop topographic cell culture devices with the confounding factors removed or well controlled. This guideline aims to promote the establishment of a highly reusable “one structural parameter – one set of cell responses” database that could facilitate the application of informatics methods, such as artificial intelligence, in the rational design of future biotopographic structures with high efficacy.
Ke Ai Publishing Communications Ltd.
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/77014/1/77014.pdf
https://doi.org/10.1016/j.bioactmat.2021.06.013
Guo, Yuanlong, Mi, Jiaomei, Ye, Chen, Ao, Yong, Shi, Mengru, Shan, Zhengjie, Li, Bingzhi, Chen, Zetao, Chen, Zhuofan, Vasilev, Krasimir, and Xiao, Yin (2022) A practical guide to promote informatics-driven efficient biotopographic material development. Bioactive Materials, 8. pp. 515-528.
https://researchonline.jcu.edu.au/77014/
open
oai:researchonline.jcu.edu.au:77016
2024-03-02T15:15:11Z
7374617475733D707562
74797065733D61727469636C65
Bacteria-Activated Dual pH- and Temperature-Responsive Hydrogel for Targeted Elimination of Infection and Improved Wound Healing
Haidari, Hanif
Vasilev, Krasimir
Cowin, Allison J.
Kopecki, Zlatko
Antibacterial treatment that provides on-demand release of therapeutics that can kill a broad spectrum of pathogens while maintaining long-term efficacy and without developing resistance or causing side effects is urgently required in clinical practice. Here, we demonstrate the development of a multistimuli-responsive hydrogel, prepared by cross-linking N-isopropylacrylamide with acrylic acid and loaded with ultrasmall silver nanoparticles (AgNPs), offering the on-demand release of Ag+ ions triggered by changes in the wound microenvironment. We demonstrate that this dual-responsive hydrogel is highly sensitive to a typical wound pH and temperature change, evidenced by the restricted release of Ag+ ions at acidic pH (<5.5) while significantly promoting the release in alkaline pH (>7.4) (>90% release). The pH-dependent release and antibacterial effect show minimal killing at pH 4 or 5.5 but dramatically activated at pH 7.4 and 10, eliminating >95% of the pathogens. The in vivo antibacterial efficacy and safety showed a high potency to clear Staphylococcus aureus wound infection while significantly accelerating the wound healing rate. This multifunctional hydrogel presents a promising bacteria-responsive delivery platform that serves as an on-demand carrier to not only reduce side effects but also significantly boost the antibacterial efficiency based on physiological needs. It offers great potential to improve the way wound infections are treated with direct clinical implications, providing a single platform for long-lasting application in wound management.
American Chemical Society
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/77016/1/77016.pdf
https://doi.org/10.1021/acsami.2c15659
Haidari, Hanif, Vasilev, Krasimir, Cowin, Allison J., and Kopecki, Zlatko (2022) Bacteria-Activated Dual pH- and Temperature-Responsive Hydrogel for Targeted Elimination of Infection and Improved Wound Healing. Applied Materials and Interfaces, 14 (46). pp. 51744-51762.
https://researchonline.jcu.edu.au/77016/
restricted
oai:researchonline.jcu.edu.au:77018
2024-03-02T15:15:14Z
7374617475733D707562
74797065733D61727469636C65
Long-term antibacterial properties of a nanostructured titanium alloy surface: An in vitro study
Bright, Richard
Fernandes, Daniel
Wood, Jonathan
Palms, Dennis
Burzava, Anouck
Ninan, Neethu
Brown, Toby
Barker, Dan
Vasilev, Krasimir
The demand for joint replacement and other orthopedic surgeries involving titanium implants is continuously increasing; however, 1%–2% of surgeries result in costly and devastating implant associated infections (IAIs). Pseudomonas aeruginosa and Staphylococcus aureus are two common pathogens known to colonise implants, leading to serious complications. Bioinspired surfaces with spike-like nanotopography have previously been shown to kill bacteria upon contact; however, the longer-term potential of such surfaces to prevent or delay biofilm formation is unclear. Hence, we monitored biofilm formation on control and nanostructured titanium disc surfaces over 21 days following inoculation with Pseudomonas aeruginosa and Staphylococcus aureus. We found a consistent 2-log or higher reduction in live bacteria throughout the time course for both bacteria. The biovolume on nanostructured discs was also significantly lower than control discs at all time points for both bacteria. Analysis of the biovolume revealed that for the nanostructured surface, bacteria was killed not just on the surface, but at locations above the surface. Interestingly, pockets of bacterial regrowth on top of the biomass occurred in both bacterial species, however this was more pronounced for S. aureus cultures after 21 days. We found that the nanostructured surface showed antibacterial properties throughout this longitudinal study. To our knowledge this is the first in vitro study to show reduction in the viability of bacterial colonisation on a nanostructured surface over a clinically relevant time frame, providing potential to reduce the likelihood of implant associated infections.
Elsevier
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/77018/1/77018.pdf
https://doi.org/10.1016/j.mtbio.2021.100176
Bright, Richard, Fernandes, Daniel, Wood, Jonathan, Palms, Dennis, Burzava, Anouck, Ninan, Neethu, Brown, Toby, Barker, Dan, and Vasilev, Krasimir (2022) Long-term antibacterial properties of a nanostructured titanium alloy surface: An in vitro study. Materials Today Bio, 13. 100176.
https://researchonline.jcu.edu.au/77018/
open
oai:researchonline.jcu.edu.au:78327
2024-02-29T14:26:45Z
7374617475733D707562
74797065733D61727469636C65
Bone Tissue Engineering Scaffolds: Function of Multi-Material Hierarchically Structured Scaffolds
Koushik, Tejas M.
Miller, Catherine M.
Antunes, Elsa
Bone tissue engineering (BTE) is a topic of interest for the last decade, and advances in materials, processing techniques, and the understanding of bone healing pathways have opened new avenues of research. The dual responsibility of BTE scaffolds in providing load-bearing capability and interaction with the local extracellular matrix to promote bone healing is a challenge in synthetic scaffolds. This article describes the usage and processing of multi-materials and hierarchical structures to mimic the structure of natural bone tissues to function as bioactive and load-bearing synthetic scaffolds. The first part of this literature review describes the physiology of bone healing responses and the interactions at different stages of bone repair. The following section reviews the available literature on biomaterials used for BTE scaffolds followed by some multi-material approaches. The next section discusses the impact of the scaffold's structural features on bone healing and the necessity of a hierarchical distribution in the scaffold structure. Finally, the last section of this review highlights the emerging trends in BTE scaffold developments that can inspire new tissue engineering strategies and truly develop the next generation of synthetic scaffolds.
Wiley-Blackwell
2023
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/78327/7/78327.pdf
https://doi.org/10.1002/adhm.202202766
Koushik, Tejas M., Miller, Catherine M., and Antunes, Elsa (2023) Bone Tissue Engineering Scaffolds: Function of Multi-Material Hierarchically Structured Scaffolds. Advanced Healthcare Materials, 12 (9). 2202766.
https://researchonline.jcu.edu.au/78327/
open
oai:researchonline.jcu.edu.au:78778
2023-05-30T23:45:46Z
7374617475733D707562
74797065733D61727469636C65
The Effect of Tropical Temperatures on the Quality of RNA Extracted from Stabilized Whole-Blood Samples
Sarathkumara, Yomani D.
Browne, Daniel J.
Kelly, Ashton M.
Pattinson, David J.
Rush, Catherine M.
Warner, Jeffrey
Proietti, Carla
Doolan, Denise L.
Whole-blood-derived transcriptional profiling is widely used in biomarker discovery, immunological research, and therapeutic development. Traditional molecular and high-throughput transcriptomic platforms, including molecular assays with quantitative PCR (qPCR) and RNA-sequencing (RNA-seq), are dependent upon high-quality and intact RNA. However, collecting high-quality RNA from field studies in remote tropical locations can be challenging due to resource restrictions and logistics of post-collection processing. The current study tested the relative performance of the two most widely used whole-blood RNA collection systems, PAXgene® and Tempus™, in optimal laboratory conditions as well as suboptimal conditions in tropical field sites, including the effects of extended storage times and high storage temperatures. We found that Tempus™ tubes maintained a slightly higher RNA quantity and integrity relative to PAXgene® tubes at suboptimal tropical conditions. Both PAXgene® and Tempus™ tubes gave similar RNA purity (A260/A280). Additionally, Tempus™ tubes preferentially maintained the stability of mRNA transcripts for two reference genes tested, Succinate dehydrogenase complex, subunit A (SDHA) and TATA-box-binding protein (TBP), even when RNA quality decreased with storage length and temperature. Both tube types preserved the rRNA transcript 18S ribosomal RNA (18S) equally. Our results suggest that Tempus™ blood RNA collection tubes are preferable to PAXgene® for whole-blood collection in suboptimal tropical conditions for RNA-based studies in resource-limited settings.
MDPI
2022
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/78778/1/78778.pdf
https://doi.org/10.3390/ijms231810609
Sarathkumara, Yomani D., Browne, Daniel J., Kelly, Ashton M., Pattinson, David J., Rush, Catherine M., Warner, Jeffrey, Proietti, Carla, and Doolan, Denise L. (2022) The Effect of Tropical Temperatures on the Quality of RNA Extracted from Stabilized Whole-Blood Samples. International Journal of Molecular Sciences, 23. 10609.
https://researchonline.jcu.edu.au/78778/
open
oai:researchonline.jcu.edu.au:79104
2024-03-03T14:40:21Z
7374617475733D696E7072657373
74797065733D61727469636C65
Electromechanical therapy in diabetic foot ulcers patients: A systematic review and meta-analysis
Rathnayake, Ayeshmanthe
Saboo, Apoorva
Vangaveti, Venkat
Malabu, Usman
Purpose: Diabetic foot ulcer (DFU) is one of the most devastating and troublesome consequences of diabetes. The current therapies are not always effective because of the complicated aetiology and interactions of local and systemic components in DFU. However, adjunctive therapy (electromechanical therapy) has become the latest modality in recent years, although there is a lack of significant research to support its utilization as a treatment standard. The purpose of this systematic research was to review the literature on the application of electromechanical therapies in the healing of DFUs.
Methods: For this systematic review, we searched PubMed, Medline, EmBase, the Cochrane library, and Google Scholar for the most current research (1990–2022) on electromechanical therapies for DFUs. We used the PICO method (where P is population, I is intervention, C is comparator/control, and O is outcome for our study) to establish research question with the terms [Electromechanical therapy OR Laser therapy OR photo therapy OR Ultrasound therapy OR Shockwave therapy] AND [diabetic foot ulcers OR diabetes] were used as search criteria. Searches were restricted to English language articles only. Whereas, Cochrane handbook of “Systematic Reviews of Interventions” with critical appraisal for medical and health sciences checklist for systematic review was used for risk of bias assessment. There were 39 publications in this study that were deemed to be acceptable. All the suitably selected studies include 1779 patients.
Results: The meta-analysis of 15 included research articles showed the overall effect was significant (P = 0.0002) thus supporting experimental groups have improvement in the DFUs healing in comparison to the control group.
Conclusion: This systematic review and meta-analysis revealed electromechanical treatments are significantly viable options for patients with DFUs. Electromechanical therapy can considerably reduce treatment ineffectiveness, accelerate healing, and minimize the time it takes for complete ulcer healing.
Springer
2023
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/79104/1/s40200-023-01240-2.pdf
https://doi.org/10.1007/s40200-023-01240-2
Rathnayake, Ayeshmanthe, Saboo, Apoorva, Vangaveti, Venkat, and Malabu, Usman (2023) Electromechanical therapy in diabetic foot ulcers patients: A systematic review and meta-analysis. Journal of Diabetes and Metabolic Disorders. (In Press)
https://researchonline.jcu.edu.au/79104/
open
oai:researchonline.jcu.edu.au:80304
2024-03-05T14:32:03Z
7374617475733D707562
74797065733D61727469636C65
Genomic testing for suspected monogenic kidney disease in children and adults: A health economic evaluation
Wu, You
Jayasinghe, Kushani
Stark, Zornitza
Quinlan, Catherine
Patel, Chirag
McCarthy, Hugh
Mallawaarachchi, Amali C.
Kerr, Peter G.
Alexander, Stephen
Mallett, Andrew J.
Goranitis, Ilias
The KidGen Collaborative investigators,
Purpose: To assess the relative cost-effectiveness of genomic testing compared with standard non-genomic diagnostic investigations in patients with suspected monogenic kidney disease from an Australian health care system perspective.
Methods: Diagnostic and clinical information was used from a national cohort of 349 participants. Simulation modelling captured diagnostic, health, and economic outcomes during a time horizon from clinical presentation until 3 months post-test results based on the outcome of cost per additional diagnosis and lifetime horizon based on cost per quality-adjusted life-year (QALY) gained.
Results: Genomic testing was Australian dollars (AU$) 1600 more costly per patient and led to an additional 27 diagnoses out of a 100 individuals tested, resulting in an incremental cost-effectiveness ratio of AU$5991 per additional diagnosis. Using a lifetime horizon, genomic testing resulted in an additional cost of AU$438 and 0.04 QALYs gained per individual compared with standard diagnostic investigations, corresponding to an incremental cost-effectiveness ratio of AU$10,823 per QALY gained. Sub-group analyses identified that the results were largely driven by the cost-effectiveness in glomerular diseases.
Conclusion: Based on established or expected thresholds of cost-effectiveness, our evidence suggests that genomic testing is very likely to be cost saving for individuals with suspected glomerular diseases, whereas no evidence of cost-effectiveness was found for non-glomerular diseases.
Nature Publishing Group
2023
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/80304/1/80304.pdf
https://doi.org/10.1016/j.gim.2023.100942
Wu, You, Jayasinghe, Kushani, Stark, Zornitza, Quinlan, Catherine, Patel, Chirag, McCarthy, Hugh, Mallawaarachchi, Amali C., Kerr, Peter G., Alexander, Stephen, Mallett, Andrew J., Goranitis, Ilias, and The KidGen Collaborative investigators, (2023) Genomic testing for suspected monogenic kidney disease in children and adults: A health economic evaluation. Genetics in Medicine, 25 (11). 100942.
https://researchonline.jcu.edu.au/80304/
open
oai:researchonline.jcu.edu.au:80321
2024-03-05T14:32:13Z
7374617475733D707562
74797065733D61727469636C65
Antimicrobial graphene-based coatings for biomedical implant applications
Romo-Rico, Jesus
Bright, Richard
Krishna, Smriti Murali
Vasilev, Krasimir
Golledge, Jonathan
Jacob, Mohan V.
Implant-associated infections (IAI) cause significant health issues and healthcare costs. In this research, we deposited graphene (Gr) on a medical-grade cobalt-chromium (CoCr) alloy surface by radiofrequency plasma-enhanced chemical vapor deposition (RF-PECVD) using Origanum vulgare as a precursor material. The deposition of Gr on the CoCr was confirmed using Raman spectroscopy and X-Ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The biocompatibility and antibacterial properties of CoCr-Gr were investigated. CoCr-Gr was biocompatible and promoted cell adhesion and spreading of RAW 267.4 macrophage cells. CoCr-Gr were antibacterial against Staphylococcus aureus and Pseudomonas aeruginosa and inhibited P. aeruginosa attachment. The results indicate that CoCr-Gr could be used as a potential antibacterial coating material for implantable devices.
Elsevier
2023
Article
PeerReviewed
application/pdf
https://researchonline.jcu.edu.au/80321/1/80321.pdf
https://doi.org/10.1016/j.cartre.2023.100282
Romo-Rico, Jesus, Bright, Richard, Krishna, Smriti Murali, Vasilev, Krasimir, Golledge, Jonathan, and Jacob, Mohan V. (2023) Antimicrobial graphene-based coatings for biomedical implant applications. Carbon Trends, 12. 100282.
https://researchonline.jcu.edu.au/80321/
open