Possible link between glycolysis and apoptosis induced by sodium fluoride
Otsuki, S., Morshed, S.R.M., Chowdhury, S.A., Takayama, F., Satoh, T., Hashimoto, K., Sugiyama, K., Amano, O., Yasui, T., Yokote, Y., Akahane, K., and Sakagami, H. (2005) Possible link between glycolysis and apoptosis induced by sodium fluoride. Journal of Dental Research, 84 (10). pp. 919-923.
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Fluoride has been used to prevent caries in the dentition, but the possible underlying mechanisms of cytotoxicity induction by this compound are still unclear. Since fluoride is known as an inhibitor of glycolytic enzymes, we investigated the possible connection between NaF-induced apoptosis and glycolysis in human promyelocytic leukemia HL-60 cells. NaF-induced apoptotic cell death is characterized by caspase activation, internucleosomal DNA fragmentation, loss of mitochondrial membrane potential, and production of apoptotic bodies. Higher activation of caspases-3 and -9, as compared with that of caspase-8, suggested the involvement of an extrinsic pathway. Utilization of glucose was nearly halted by NaF, whereas that of glutamine was rather enhanced. NaF enhanced the expression of Bad protein, but not that of Bcl-2 and Bax proteins, and reduced HIF-1 mRNA expression. Analysis of these data suggests a possible link between glycolysis and apoptosis.
|Item Type:||Article (Refereed Research - C1)|
Copyright © 2005, IADR Journals Online by International & American Associations for Dental Research.
|Keywords:||apoptosis, glycolysis, bad protein, fluoride|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry @ 0%|
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism @ 0%
|Deposited On:||01 Nov 2006|
|Last Modified:||26 Aug 2013 15:32|
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