Purification of immunoglobulins from chicken sera by thiophilic gel chromatography
Constantinoiu, C.C., Molloy, J.B., Jorgensen, W.K., and Coleman, G.T. (2007) Purification of immunoglobulins from chicken sera by thiophilic gel chromatography. Poultry Science, 86 (9). pp. 1910-1914.
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Immunoglobulin Y is different from most of the other immunoglobulins because it does not bind protein A or protein G. Thiophilic gel chromatography has been successfully used to purify IgY from chicken egg yolk, but the technology has not previously been used to purify IgY from serum. In this research note, we describe the optimization of T-gel chromatography for purification of IgY from serum. Data are provided on the recovery and purity of IgY obtained using potassium sulfate buffers of different concentrations. Decreasing the strength of potassium sulfate buffer from 0.5 to 0.3 M did not alter the amount of IgY recovered but increased the purity. Using 0.3 M potassium sulphate, we recovered approximately 63.7% of the serum Ig as almost pure IgY.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||immunoglobulin Y, serum, purification, thiophilic chromatography|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070705 Veterinary Immunology @ 50%|
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 50%
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%|
|Deposited On:||28 Apr 2010 14:38|
|Last Modified:||12 Feb 2011 23:22|
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