The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Abstract

Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, losely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.