The assessment of TGGE for the detection of interspecific and intergeneric DNA-marker polymorphism within Solanaceae
Garland, S.H. (2005) The assessment of TGGE for the detection of interspecific and intergeneric DNA-marker polymorphism within Solanaceae. Australian Journal of Agricultural Research, 56 (3). pp. 291-299.
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View at Publisher Website: http://dx.doi.org/10.1071/AR04141
RFLP markers are currently the most appropriate marker system for the identification of uncharacterised polymorphism at the interspecific and intergeneric level. Given the benefits of a PCR based marker system and the availability of sequence information for many Solanaceous cDNA clones, it is now possible to target conserved fragments, for primer development, that flank sequence possessing interspecific polymorphism. The potential outcome is the development of a suite of markers that amplify widey in Solanaceae. Temperature gradient gel electrophoresis (TGGE) is a relatively inexpensive gel based system that is suitable for the detection of most single-base changes. TGGE can be used to screen for both known and unknown polymorphisms, and has been assessed here, for the development of PCR based markers that are useful for the detection of interspecific variation within Solanaceae. Fifteen markers are presented where differences between Lycopersicon esculentum and L. pennellii have been detected by TGGE. The markers were assessed on a wider selection of plant species and found to be potentially useful for the identification of interspecific and intergeneric polymorphism in Solanaceous plants.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||tomato; potato; Solanum; capsicum, pepper|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology @ 100%|
|SEO Codes:||82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8202 Horticultural Crops > 820215 Vegetables @ 100%|
|Deposited On:||15 Mar 2010 13:24|
|Last Modified:||25 May 2013 01:09|
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