Expression of Hc subunits from Clostridium botulinum types C and D and their evaluation as candidate vaccine antigens in mice
Woodward, L.A., Arimitsu, H., Hirst, R., and Oguma, K. (2003) Expression of Hc subunits from Clostridium botulinum types C and D and their evaluation as candidate vaccine antigens in mice. Infection and Immunity, 71 (5). pp. 2941-2944.
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Two proteins representing the heavy-chain subunits of botulinum neurotoxin types C and D were expressed in Escherichia coli, and their vaccine potential was evaluated. Mice were vaccinated with doses ranging from 0.5 to 10 µg and were challenged with 10 to 105 50% lethal doses of toxin. For the type C subunit protein, C50, two doses of 2 µg were required for full protection, while, for type D subunit protein, D50, two 1-µg doses were required. A bivalent vaccine consisting of a mixture of these two proteins also provided protection against both botulinum neurotoxin type C and type D challenge. Antibody levels in serum were determined by both enzyme-linked immunosorbent assays and serum neutralization assays.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||antigens; botulism; Clostridium|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070205 Animal Protection (Pests and Pathogens) @ 100%|
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830301 Beef Cattle @ 100%|
|Deposited On:||25 Jan 2010 13:04|
|Last Modified:||17 Jun 2013 00:55|
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|Citation Counts with External Providers:||Web of Science: 28|
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