Onchocerca volvulus: expression and immunolocalization of a nematode cathepsin D-loike lysosomal aspartic protease
Jolodar, Abbas, Fischer, Peter, Büttner, Dietrich W., Miller, David J., Schmetz, Christel, and Brattig, Norbert W. (2004) Onchocerca volvulus: expression and immunolocalization of a nematode cathepsin D-loike lysosomal aspartic protease. Experimental Parasitology, 107 (3-4). pp. 145-156.
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The N-terminal region of the cathepsin D-like aspartic protease from the human filarial parasite Onchocerca volvulus was expressed as His-tag fusion protein. Light and electron microscopic immunohistology using antibodies against the recombinant protein showed labeling of lysosomes in the hypodermis and epithelia of the intestine and the reproductive organs of Onchocerca. While developing oocytes were negative, mature oocytes and early morulae showed strong labeling. In older embryos and mature microfilariae, stained lysosomes were only found in a few cells. Cell death in degenerating microfilariae of patients untreated and treated with microfilaricidal drugs was associated with strong expression of aspartic protease. IgG1, IgG4, and IgE antibodies reactive with the recombinant protein were demonstrated in sera from onchocerciasis patients indicating exposure and recognition of the enzyme by the host’s defence system. The aspartic protease of O. volvulus appears to function in intestinal digestion and tissue degradation of the filaria.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||aspartic protease; embryogenesis; filaria; lysosomes; Nematoda|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%|
|Deposited On:||17 Feb 2010 15:52|
|Last Modified:||20 May 2013 00:53|
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|Citation Counts with External Providers:||Web of Science: 23|
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