Techniques to kill infective larvae of human hookworm Necator americanus in the laboratory and a new material safety data sheet
Speare, Rick, Melrose, Wayne, Cooke, Sharon, and Croese, John (2008) Techniques to kill infective larvae of human hookworm Necator americanus in the laboratory and a new material safety data sheet. Australian Journal of Medical Sciences, 29 (3). pp. 91-96.
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With increasing experimental use of the human hookworm Necator americanus in therapeutic trials, an evidence based approach to laboratory safety is needed to produce an accurate Material Safety Data Sheet (MSDS). Five chemical disinfectants and hot water were evaluated for their killing effect on infective larvae (iL3) of N. americanus. The most effective chemical was 70% ethanol which achieved 100% kill in 10 min. Dettol® was also effective after 20 min. Formalin (10% concentration) had a slow killing effect while bleach (1% concentration) and glutaraldehyde (2% concentration) were ineffective. Infective larvae were killed by hot water at 80 oC or higher. In a simulated bench top spill 70% ethanol at five times the spill volume was 100% effective after 20 min. Addition of hot water was effective when the water was close to boiling, but did not kill 100% of iL3 at 80 oC.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Necator americanus; hookworm; MSDS; Material Safety Data Sheet; laboratory safety|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 100%|
|SEO Codes:||92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 80%|
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920105 Digestive System Disorders @ 20%
|Deposited On:||15 Feb 2010 09:15|
|Last Modified:||13 Feb 2011 03:53|
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