Development and characterization of eight new microsatellite markers for the haremic sandperch, Parapercis cylindrica (family Pinguipedidae)

Smith-Keune, C., Jerry, D.R., Evans, B.S., McCormick, M.I., Munday, P.L., and Jones, G.P. (2006) Development and characterization of eight new microsatellite markers for the haremic sandperch, Parapercis cylindrica (family Pinguipedidae). Molecular Ecology Resources, 6 (4). pp. 1036-1038.

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DOI: 10.1111/j.1471-8286.2006.01421.x

View at Publisher Website: http://dx.doi.org/10.1111/j.1471-8286.20...

Abstract

Eight di-, tri- and tetranucleotide microsatellite markers were developed for the haremic sandperch Parapercis cylindrica using a linker-ligated, magnetic bead enrichment protocol. Screening of at least 17 individuals showed these markers to be polymorphic with observed heterozygosity ranging from 0.381 to 1.000 (mean = 0.742) and the numbers of alleles ranging from three to 18. The average polymorphic information content for these eight loci was 0.723. These markers may be used for parentage studies aimed at exploring the complex mating strategies employed by this haremic coral reef fish and will be valuable for population genetic studies.

ID Code:5201
Item Type:Article (Refereed Research - C1)
Keywords:enrichment; microsatellites; parentage; population genetics; reef fish
FoR Codes:06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 80%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 20%
SEO Codes:97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Deposited On:04 Sep 2009 10:29
Last Modified:22 May 2013 00:44
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