Paragonimus heterotremus Chen and Hsia (1964), in Vietnam: A molecular identification and relationships of isolates from different hosts and geographical origins
Le, Thanh, Van De, Nguyen, Blair, David, McManus, Donald P., Kino, Hideto, and Agatsuma, Takeshi (2006) Paragonimus heterotremus Chen and Hsia (1964), in Vietnam: A molecular identification and relationships of isolates from different hosts and geographical origins. Acta Tropica, 98 (1). pp. 25-33.
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Paragonimus heterotremus Chen and Hsia (1964), and paragonimiasis caused by this species is a newly detected disease in Vietnam. Twelve samples of Paragonimus (Platyhelminthes: Trematoda: Digenea: Paragonimidae) from different life-stages (eggs, miracidia, metacercariae, adults from natural and experimental hosts) and host species (crab, dog, cat and human) were collected in different geographical locations in Vietnam. DNA sequences were obtained from each for partial mitochondrial cytochrome c oxidase subunit 1 (cox1) (387 bp) and the entire second ribosomal internal transcribed spacer (ITS-2) (361 bp). The ITS-2 sequences were identical among all specimens, including those previously reported in GenBank. For cox1, there were sequence differences between specimens from Vietnam (four provinces, different locations) and those from Guangxi (China) and Saraburi (Thailand). Phylogenetic trees inferred from cox1 and ITS-2 sequences using sequence data for 15 P. heterotremus and for other Paragonimus spp. revealed that all P. heterotremus originating from Vietnam, Thailand and China form a distinct group. This information also confirms the identity of the Vietnamese specimens as P. heterotremus.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||mitochondrial DNA; heterotremus; cytochrome c oxidase subunit 1; internal transcribed spacer-2(ITS-2); metacerariae; identification; phylogeny|
|FoR Codes:||10 TECHNOLOGY > 1099 Other Technology > 109999 Technology not elsewhere classified @ 100%|
|SEO Codes:||92 HEALTH > 9202 Health and Support Services > 920203 Diagnostic Methods @ 100%|
|Deposited On:||23 Nov 2009 11:22|
|Last Modified:||18 Oct 2013 00:33|
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