Protein cross-contamination during batch cleaning and autoclaving of the ProSeal laryngeal mask airway

Abstract

We tested the hypothesis that protein cross-contamination occurs during batch cleaning and autoclaving of a reusable extraglottic airway device, the ProSeal™ laryngeal mask airway. At the end of each day for 10 days, nine laryngeal mask airways that had been used for non-intra-oral surgery were cleaned and autoclaved alongside a new unused laryngeal mask airway. In addition, a new unused laryngeal mask airway underwent the same cleaning and autoclaving procedures in isolation. Protein staining was more frequently detected on the unused laryngeal mask airways that were processed by batch rather than in isolation (p < 0.01). Protein staining was detected on all unused laryngeal mask airways that were processed by batch, but none on those processed in isolation. Protein staining was more severe with the used compared with the unused laryngeal mask airways (p < 0.001). We conclude that protein cross-contamination of the laryngeal mask airway occurs during batch cleaning and autoclaving and recommend that reusable airway devices are cleaned in isolation.