A genetic analysis of the Drosophila mcm5 gene defines a domain specifically required for meiotic recombination
Lake, Cathleen M., Teeter, Kathy, Page, Scott L., Nielsen, Rachel, and Hawley, R. Scott (2007) A genetic analysis of the Drosophila mcm5 gene defines a domain specifically required for meiotic recombination. Genetics, 176 (4). pp. 2151-2163.
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Members of the minichromosome maintenance (MCM) family have pivotal roles in many biological processes. Although originally studied for their role in DNA replication, it is becoming increasingly apparent that certain members of this family are multifunctional and also play roles in transcription, cohesion, condensation, and recombination. Here we provide a genetic dissection of the mcm5 gene in Drosophila that demonstrates an unexpected function for this protein. First, we show that homozygotes for a null allele of mcm5 die as third instar larvae, apparently as a result of blocking those replication events that lead to mitotic divisions without impairing endo-reduplication. However, we have also recovered a viable and fertile allele of mcm5 (denoted mcm5^sup A7^) that specifically impairs the meiotic recombination process. We demonstrate that the decrease in recombination observed in females homozygous for mcm5^sup A7^ is not due to a failure to create or repair meiotically induced double strand breaks (DSBs), but rather to a failure to resolve those DSBs into meiotic crossovers. Consistent with their ability to repair meiotically induced DSBs, flies homozygous for mcm5^sup A7^ are fully proficient in somatic DNA repair. These results strengthen the observation that members of the prereplicative complex have multiple functions and provide evidence that mcm5 plays a critical role in the meiotic recombination pathway.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Drosophila; meiotic recombination; mcm5 gene; genetic analysis|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060402 Cell and Nuclear Division @ 50%|
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation @ 50%
|SEO Codes:||92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920114 Reproductive System and Disorders @ 100%|
|Deposited On:||23 Jul 2009 17:28|
|Last Modified:||13 May 2013 00:27|
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|Citation Counts with External Providers:||Web of Science: 12|
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