Production of polyclonal and monoclonal antibodies against gill-associated virus and the development of an ELISA
Munro, James, and Owens, Leigh (2007) Production of polyclonal and monoclonal antibodies against gill-associated virus and the development of an ELISA. Aquaculture, 262 (2-4). pp. 173-182.
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Gill-associated virus (GAV) is the type species of the genus Okavirus in the Roniviridae of the Nidovirales and has been associated with mortalities in farmed Penaeus monodon (black tiger prawn). Polyclonal antibodies (PAbs) and monoclonal antibodies (MAbs) specific to GAV were produced following immunisation with protein subunits of purified GAV isolated from the gill tissues of P. monodon into either a six week old chicken (PAbs) or into a six week old Balb/c mouse for the production of the MAbs. Western blot analysis indicated that the PAbs reacted with 63, 110 and 170 kDa proteins, while the MAbs reacted to the 20 kDa protein of GAV. The MAbs were further investigated to ensure specificity by immunohistochemistry and elution of affinity purified GAV protein from the MAbs. An enzyme linked immunosorbent assay was then developed using the prepared PAbs and MAbs for the detection of GAV.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||gill-associated virus; yellowhead virus; Elisa; detection method; antibodies|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 100%|
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%|
|Deposited On:||22 Jul 2009 09:16|
|Last Modified:||18 Oct 2013 00:26|
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