The domains carrying the opposing activities in adenylyltransferase are separated by a central regulatory domain
Clancy, Paula, Xu, Yibin, van Heeswijk, Wally C., Vasudevan, Subhash G., and Ollis, David L. (2007) The domains carrying the opposing activities in adenylyltransferase are separated by a central regulatory domain. FEBS journal, 274 (11). pp. 2865-2877.
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Adenylyltransferase is a bifunctional enzyme that controls the enzymatic activity of dodecameric glutamine synthetase in Escherichia coli by reversible adenylylation and deadenylylation. Previous studies showed that the two similar but chemically distinct reactions are carried out by separate domains within adenylyltransferase. The N-terminal domain carries the deadenylylation activity, and the C-terminal domain carries the adenylylation activity [Jaggi R, van Heeswijk WC, Westerhoff HV, Ollis DL & Vasudevan SG (1997) EMBO J16, 5562-5571]. In this study, we further map the domain junctions of adenylyltransferase on the basis of solubility and enzymatic analysis of truncation constructs, and show for the first time that adenylyltransferase has three domains: the two activity domains and a central, probably regulatory (R), domain connected by interdomain Q-linkers (N-Q1-R-Q2-C). The various constructs, which have the opposing domain and or central domain removed, all retain their activity in the absence of their respective nitrogen status indicator, i.e. PII or PII-UMP. A panel of mAbs to adenylyltransferase was used to demonstrate that the cellular nitrogen status indicators, PII and PII-UMP, probably bind in the central regulatory domain to stimulate the adenylylation and deadenylylation reactions, respectively. In the light of these results, intramolecular signaling within adenylyltransferase is discussed.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||glutamine synthetase; E. coli; nitrogen; metabolism; adenyltransferase; intramolecular signaling; monoclonal antibody; regulatory domain|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE @ 100%|
|Deposited On:||09 Jun 2009 15:04|
|Last Modified:||16 Jun 2013 00:25|
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|Citation Counts with External Providers:||Web of Science: 8|
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