Screening marine fungi for inhibitors of the C4 plant enzyme pyruvate phosphate dikinase: unguinol as a potential novel herbicide candidate
Motti, Cherie A., Bourne, David, Burnell, James N., Doyle, Jason R., Haines, Dianne S., Liptrot, Catherine H., Llewellyn, Lyndon E., Ludke, Shilo, Muirhead, Andrew, and Tapiolas, Dianne M. (2007) Screening marine fungi for inhibitors of the C4 plant enzyme pyruvate phosphate dikinase: unguinol as a potential novel herbicide candidate. Applied and Environmental Microbiology, 73 (6). pp. 1921-1927.
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View at Publisher Website: http://dx.doi.org/10.1128/AEM.02479-06
A total of 2,245 extracts, derived from 449 marine fungi cultivated in five types of media, were screened against the C4 plant enzyme pyruvate phosphate dikinase (PPDK), a potential herbicide target. Extracts from several fungal isolates selectively inhibited PPDK. Bioassay-guided fractionation of one isolate led to the isolation of the known compound unguinol, which inhibited PPDK with a 50% inhibitory concentration of 42.3 ± 0.8 µM. Further kinetic analysis revealed that unguinol was a mixed noncompetitive inhibitor of PPDK with respect to the substrates pyruvate and ATP and an uncompetitive inhibitor of PPDK with respect to phosphate. Unguinol had deleterious effects on a model C4 plant but no effect on a model C3 plant. These results indicate that unguinol inhibits PPDK via a novel mechanism of action which also translates to an herbicidal effect on whole plants.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||PPDK; herbicides; marine fungi; screening|
|FoR Codes:||10 TECHNOLOGY > 1099 Other Technology > 109999 Technology not elsewhere classified @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE @ 100%|
|Deposited On:||28 May 2009 12:57|
|Last Modified:||20 May 2013 00:23|
Last 12 Months: 0
|Citation Counts with External Providers:||Web of Science: 9|
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