Ballagh, D.A., Pankhurst, P.M., and Fielder, D.S. (2011) Embryonic development of mulloway, Argyrosomus japonicus, and egg surface disinfection using ozone. Aquaculture, 318 (3-4). pp. 475-478.

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Abstract

The embryonic development of mulloway, Argyrosomus japonicus, was described. Ozone disinfection procedures were investigated using the combined effects of ozone CT (concentration of ozone [mg l−1] multiplied by contact time [min]; 0, 0.1, 0.5, 1 or 5) and treatment temperature (19, 22 or 25 °C), as well as the effect of ozone exposure (CT = 1) when applied at four different stages of embryonic development (3, 8, 20 or 27 h post fertilisation; HPF) on the hatching success of mulloway larvae. Significantly fewer eggs hatched when treated with a CT of 5 compared with those treated with a CT of 0, 0.1, 0.5 or 1, particularly when eggs were maintained in water of 19 °C. There were no apparent negative impacts of treating eggs at 22 or 25 °C and the highest CT value that did not negatively affect hatching was a CT of 1; therefore, it is recommended that eggs should be ozone treated with a CT of 1 at 22 °C. There were no significant effects of ozone treatment (CT = 1) on hatchability of eggs when applied at any of the development stages examined.

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