Identification of twenty one microsatellite loci for conservation genetic studies of the endemic butterflyfish Chaetodon tricinctus
van der Meer, Martin H., Gardiner, Michael G., Hobbs, Jean-Paul A., Pratchett, Morgan, and van Herwerden, Lynne (2012) Identification of twenty one microsatellite loci for conservation genetic studies of the endemic butterflyfish Chaetodon tricinctus. Conservation Genetics, 4 (2). pp. 243-246.
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Coral reef habitats are increasingly under threat from global warming and this has influenced the associated reef fish communities. Chaetodon tricinctus is a butterflyfish endemic to the offshore reefs of Elizabeth Reef, Middleton Reef, Lord Howe Island and Norfolk Island off Australia's east coast. It is a highly specialised coral reef fish that is thought to rely on Acroporid coral species exclusively for food and shelter. We developed primers for twenty one microsatellite loci to reveal gene flow, population genetic structure and genetic diversity within and among these three reefs. Observed heterozygosities ranged from 0.185 to 0.964 and expected heterozygosities ranged from 0.230 to 0.889 in 30 individuals from Middleton Reef. When cross tested with Chaetodon trifascialis, a closely related species, there was poor amplification success and only a moderate level of polymorphism. Therefore, although these loci will be useful in C. tricinctus, it is unlikely that they can be used on other related butterflyfishes.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||coral reef fish, island endemics, genetic diversity, cross-species amplification|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%|
|Deposited On:||16 Jan 2012 14:52|
|Last Modified:||30 Jul 2013 01:13|
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