Proteomic characterisation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans
Aziz, Ammar, Zhang, Wenbao, Li, Jun, Loukas, Alex, McManus, Donald, and Mulvenna, Jason (2011) Proteomic characterisation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans. Journal of Proteomics, 74 (9). pp. 1560-1572.
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The hydatid cyst fluid (HCF) of Echinococcus granulosus is a complex biological mixture containing a wide range of proteins of both parasite and host origin. Using a combination of in- and off-gel protein fractionation techniques and tandem mass spectrometry 130 HCF proteins were identified from fertile cysts of sheep and human origin and infertile cysts from cattle. Forty-eight proteins were of parasite origin including Antigen 5 and Antigen B - the most abundant parasite proteins, thioredoxin, low-density lipoprotein receptors, cyclophilin and ferritin. Across the three host species the identified HCF proteins were broadly similar although, based on spectral counts, three proteins, including an antigen B isoform, were more abundant in sheep HCF compared with the fluids of cattle and human origin. Eighty-two host proteins were identified in HCF from the three species. Host plasma proteins were the most abundant, although approximately thirty of the host proteins that were identified are not considered constituents of plasma. The identification of parasite heat shock proteins and annexin A13 exclusively in infertile cysts, along with an increased spectral count for cathepsin B, supports the hypothesis of increased cellular stress and apoptosis as the cause of their infertility.
|Item Type:||Article (Refereed Research - C1)|
Supplemental material available at http://dx.doi.org/10.1016/j.jprot.2011.02.021
|Keywords:||Echinococcus granulosus, proteomics, excretory/secretory, secretory proteins, hydatid cyst|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%|
|SEO Codes:||92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%|
|Deposited On:||05 Dec 2011 16:00|
|Last Modified:||23 May 2013 01:45|
Last 12 Months: 0
|Citation Counts with External Providers:||Web of Science: 8|
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