Paired-ion extraction and high-performance liquid chromatographic determination of diminazine in cattle plasma: a modified method
Gummow, B., Du Preez, J.L., and Swann, G.E. (1995) Paired-ion extraction and high-performance liquid chromatographic determination of diminazine in cattle plasma: a modified method. Onderstepoort Journal of Veterinary Research, 62 . pp. 1-4.
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The high-performance liquid chromatographic method published by Aliu & Odegaard (1983) was found to give poor peak separation when used to determine plasma diminazene concentrations in cattle. Before bioequivalence studies could be carried out, the method had to be modified. Solid-phase extraction with acetonitrile/0.025 M Na-octane sulphonate and 2% acetic acid as eluent, followed by sample concentration, gave recoveries of > 90% for diminazene and the internal standard. A mobile phase of acetonitrile/0,005 M Na-octane sulphonate, 0.1% triethylamine, pH 3.2 with acetic acid on a Nova Pak C18 column was used for the analysis. Wavelength switching was used to determine the internal standard (imidocarb) and diminazene at their respective wavelengths of maximum absorbance, resulting in a fivefold increase in the limit of detection for diminazene. The modified method attained a detection limit of 2 ng.ml-1 (peak 4x baseline noise), limit of quantitation of 10 ng.ml-1 (coefficient of variation < 15%) and an accuracy of > 96% over the range from 10-5000 ng.ml-1.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||paired-ion extraction, liquid chromatographic determination, diminazene, cattle plasma|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070704 Veterinary Epidemiology @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%|
|Deposited On:||06 Jun 2012 13:22|
|Last Modified:||06 Jun 2012 18:01|
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