Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)
Waycott, Michelle, Jones, Brendan L., Van Dijk, Jent Kornelis, Robson, Heather L.A., and Calladine, Ainsley (2011) Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae). American Journal of Botany, 98 (4). e81-e83.
|PDF (Published Version) - Repository staff only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader|
View at Publisher Website: http://dx.doi.org/10.3732/ajb.1000356
Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified.
Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14.
Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||microsatellite; polyploidy; Solanum centrale|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060703 Plant Developmental and Reproductive Biology @ 10%|
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060207 Population Ecology @ 30%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 60%
|SEO Codes:||96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960504 Ecosystem Assessment and Management of Farmland, Arable Cropland and Permanent Cropland Environments @ 100%|
|Deposited On:||26 Sep 2011 15:20|
|Last Modified:||11 May 2013 01:35|
Last 12 Months: 0
|Citation Counts with External Providers:||Web of Science: 3|
Repository Staff Only: item control page