Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii: development from 454-sequence data and utility in related taxa
Todd, Erica, Blair, David, Hamann, Mark, and Jerry, Dean (2011) Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii: development from 454-sequence data and utility in related taxa. Conservation Genetics Resources, 3 (3). pp. 449-456.
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454 shotgun sequencing was used to identify thousands of in silico microsatellite loci from two Australian freshwater turtle species (Pleurodira, Chelidae), Elseya albagula and Emydura macquarii krefftii. Twenty-nine polymorphic microsatellites were subsequently developed and tested as markers, 15 for E. m. krefftii and 14 for E. albagula. The former marker set showed greater allelic richness (mean N A = 7.7, range 3–15) than the latter (mean N A = 4.4, range 2–8). Levels of expected heterozygosity were high for both marker suites (mean H E = 0.718, range 0.177–0.875; and mean H E = 0.567, range 0.317–0.827, respectively), indicating both are suitable for studies of population genetic structure, connectivity and mating systems in these taxa. Cross-species amplification in 12 other short-necked freshwater turtle taxa highlighted the potential utility of these markers in other species, notably Emydura spp., Rheodytes leukops and Elusor macrurus.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Testudines, Chelidae, 454 pyrosequencing, multiplex PCR, gene flow, river regulation|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 50%|
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 50%
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830102 Aquaculture Fin Fish (excl. Tuna) @ 100%|
|Deposited On:||18 Aug 2011 16:13|
|Last Modified:||18 Oct 2013 01:20|
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