A short, reliable, highly reproducible complement fixation test for contagious equine metritis
Gummow, B., Herr, S., and Brett, O.L. (1986) A short, reliable, highly reproducible complement fixation test for contagious equine metritis. Onderstepoort Journal of Veterinary Research, 53 . pp. 241-243.
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A complement fixation test, using round~bottomed microtitration plates and an 8 channel microdiluter, based on that used for brucellosis by Herr, Huchzenneyer, Te Brugge, Williamson, Roos & Schiele, 1985, has been developed for use on the sera of horses to detect antibodies to the contagious equine metritis organism. The results with 2 known positive sera tested 116 times in 27 separate tests were reproducible for the most part within a twofold range. They seldom exceeded these limits and never' exceeded a fourfold range. The test itself is capable of being carried out within 90 min. The test was slightly more sensitive when sera were inactivated in a hot air oven for 50 min at 58 °C, as compared to inactivation at 62 °C in a water-bath for 50 min. There were no false negative or false positive reactions and no anticomplementary activity in the sera tested.
|Item Type:||Article (Refereed Research - C1)|
|FoR Codes:||07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070704 Veterinary Epidemiology @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%|
|Deposited On:||07 Jun 2012 10:36|
|Last Modified:||07 Jun 2012 18:02|
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