Cross-species amplification of 44 microsatellite loci developed for Chaetodon trifascialis, C. lunulatus and C. vagabundus in 22 related butterflyfish species
Lawton, Rebecca J., Pratchett, Morgan S., and Bay, Line K. (2011) Cross-species amplification of 44 microsatellite loci developed for Chaetodon trifascialis, C. lunulatus and C. vagabundus in 22 related butterflyfish species. Molecular Ecology Resources, 11 (2). pp. 323-327.
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Forty-four microsatellite primers developed for three species of butterflyfish were cross-tested against 22 related confamilial species. Amplification success and cross-species transferability of these markers were moderately high. Between 24 and 37 loci were amplified successfully in each species, with a mean success rate per species of 71.7% (± 1.8 SE). Rates of amplification success were comparable among primers designed for the three source species, ranging from a mean success rate per species of 16.9 loci (± 0.8 SE) for Chaetodon trifascialis source loci to 13.7 loci (± 1.5 SE) for C. vagabundus source loci. Polymorphism rates were high (76.1%± 3.1 SE of all successfully amplified loci), and 10 loci were polymorphic in all successfully amplified species (Tri14, B11, C5, D3, D113, D6, D117, D120, D111, D118). The number of alleles per polymorphic locus ranged from 2 to 8, and the average number of alleles across all polymorphic loci and all species was 3.6 (± 0.07 SE). Polymorphism rates were higher overall in primers designed for C. vagabundus (89.9%± 3.9 SE). Overall cross-testing success was lowest for Heniochus chrysostomus, the most phylogenetically divergent species. The significant cross-testing reported here provides a valuable resource that will enable population genetics studies to be undertaken on a range of butterflyfishes without the need for expensive and time-consuming de novo microsatellite development.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Chaetodontidae; coral reef fishes; cross-species amplification; ecological specialization; microsatellite primer; population genetics|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%|
|SEO Codes:||96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%|
|Deposited On:||15 Jun 2011 14:19|
|Last Modified:||18 Oct 2013 01:15|
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