Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells

Sahle, M., Burgess, W.G., and Kideanemariam, A. (2002) Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells. Onderstepoort Journal of Veterinary Research, 69 (3). pp. 201-206.

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Abstract

This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran were incorporated in Dulbecco's modified Eagle's medium to encourage secondary viral multiplication without adversely affecting healthy Madin Derby bovine kidney cell growth.

ID Code:13652
Item Type:Article (Refereed Research - C1)
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Keywords:cell culture; MDBK cell; Newcastle disease virus; V4 strain replication
FoR Codes:07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070205 Animal Protection (Pests and Pathogens) @ 100%
SEO Codes:83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830309 Poultry @ 100%
Deposited On:06 Dec 2010 17:04
Last Modified:12 Feb 2011 03:59
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