A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining
Green, B.S., Reilly, S.M., and McCormick, M.I. (2002) A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining. Journal of Fish Biology, 61 (6). pp. 1600-1605.
|PDF (Published Version) - Repository staff only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader|
View at Publisher Website: http://dx.doi.org/10.1111/j.1095-8649.20...
A fast and cost-effective method for examining otoliths in fish larvae was developed whereby the otolith remains in situ. Whole fish of the clownfish Amphiprion melanopus were enzymecleared using a laundry pre-soak and then stained using the Von Kossa silver staining method for calcium. The otolith nucleus, daily rings and the otolith edge were all clearly visible and were suitable for a variety of age and growth analyses. The total ‘hands on’ time required to process these otoliths was c. 3 min, and multiple samples could be processed simultaneously. The reduction in labour of this method to produce clear daily rings in the otolith lends itself to broad use in fish biology where large quantities of otoliths need to be examined in a cost- and time-efficient manner.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Amphiprion melanopus; daily increments; fish larvae; otoliths|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%|
|Deposited On:||08 Dec 2010 15:50|
|Last Modified:||12 Feb 2011 20:19|
Last 12 Months: 0
Repository Staff Only: item control page