Identification of the amino acid residues responsible for cold tolerance in Flaveria brownii pyruvate,orthophosphate dikinase
Ohta, Shozo, Usami, Satoru, Ueki, Jun, Kumashiro, Takashi, Komar, Toshihiko, and Burnell, Jim N. (1996) Identification of the amino acid residues responsible for cold tolerance in Flaveria brownii pyruvate,orthophosphate dikinase. FEBS Letters, 396 (2). pp. 152-156.
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Pyruvate,orthophosphate dikinase (PPDK), an enzyme important in C4 photosynthesis, is typically a cold-sensitive enzyme. However, a cold-tolerant form of the enzyme has been isolated from the leaves of Flaveria brownii. Using an Escherichia coli expression system and the PPDK cDNAs from F. brownii (cold-tolerant), F. bidentis (cold-sensitive) and maize (intermediate cold tolerance), site-directed mutagenesis studies indicated that as few as three amino acids residues (of 880 residues) strongly influence the cold sensitivity of Flaveria PPDK. Gel filtration analysis of the PPDK expressed in E. coli showed that subunit association and cold tolerance are closely linked.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Pyruvate,orthophosphate dikinase; C4 photosynthesis; Chilling; Flaveria|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%|
|Deposited On:||13 Oct 2010 13:28|
|Last Modified:||12 Feb 2011 20:56|
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