In vitro fertilization efficiency in Acropora digitifera
Iguchi, Akira, Morita, Masaya, Nakajima, Yuichi, Nishikawa, Akira, and Miller, David (2009) In vitro fertilization efficiency in Acropora digitifera. Zygote, 17 . pp. 225-227.
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We performed fertilization experiments with Acropora digitifera, which is one of the dominant scleractinian corals in the Ryukyu Archipelago, Japan, to determine optimal conditions for in vitro manipulations. Our result suggests that conspecific fertilization is essentially complete within 30 min under the experimental conditions used in usual fertilization experiments in corals. Previous in vitro experiments (1 × 10^5– 10^6 sperm/ml, 4–8 h) are likely to have overestimated the efficiency of fertilization of Acropora spp. in the field. Therefore, we suggest that incubation periods shorter than those used to date (i.e. complete exclusion of sperm 1 h after their addition) would be more appropriate for the estimation of fertilization rates in corals.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Acropora digitifera; coral; in vitro fertilization|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0608 Zoology > 060803 Animal Developmental and Reproductive Biology @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%|
|Deposited On:||11 May 2010 10:23|
|Last Modified:||18 Oct 2013 01:02|
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