An in vivo comparison of the efficacy of CSL box jellyfish antivenom with antibodies raised against nematocyst-derived Chironex fleckeri venom
Winter, Kelly L., Isbister, Geoffrey K., Jacoby, Tamara, Seymour, Jamie E., and Hodgson, Wayne C. (2009) An in vivo comparison of the efficacy of CSL box jellyfish antivenom with antibodies raised against nematocyst-derived Chironex fleckeri venom. Toxicology Letters, 187 (2). pp. 94-98.
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Although CSL box jellyfish antivenom (AV) remains the primary treatment for Chironex fleckeri envenoming, there has been considerable debate regarding its clinical effectiveness. Animal studies have shown that AV is largely ineffective in preventing C. fleckeri-induced cardiovascular collapse. This study examined the effectiveness of CSL box jellyfish AV (ovine IgG), raised against ‘milked’ venom, and polyclonal rabbit IgG antibodies (Ab) raised against nematocyst-derived venom. A venom dose of 30 μg/kg, i.v., which causes an initial presser response (34 ± 5 mmHg; n = 7) followed by cardiovascular collapse, was used in all experiments. A bolus dose of AV (3000 U/kg, i.v.) or Ab (12 mg; i.e. an equivalent protein ‘load’ to 3000 U/kg AV), administered 15 min prior to a bolus dose of venom, did not significantly attenuate the effects of venom. The venom response was also not significantly attenuated when AV (3000 U/kg) was given as a bolus dose 10–60 min prior to venom infusion. However, when the venom was incubated with either AV (3000 U/kg) or Ab (12 mg) for 3 h prior to infusion, the effect of the venom was almost abolished. The results of this study demonstrate that antibodies raised against both ‘milked’ and nematocyst-derived venom are able to neutralise the cardiovascular collapse produced by the venom. However, large amounts of AV are required and must be preincubated with the venom to be protective. This indicates a very rapid action of the toxin(s) and that AV is unlikely to be clinically effective because it cannot be administered early enough.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||venom, jellyfish, antivenom, antibodies, cardiovascular, envenoming, tropical biology|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0602 Ecology > 060299 Ecology not elsewhere classified @ 100%|
|SEO Codes:||96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%|
|Deposited On:||08 May 2010 11:00|
|Last Modified:||30 Apr 2013 01:18|
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|Citation Counts with External Providers:||Web of Science: 6|
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