High levels of intra-specific variation in the NG repeat region of the Pinctada maxima N66 organic matrix protein
Smith-Keune, Carolyn, and Jerry, Dean R. (2009) High levels of intra-specific variation in the NG repeat region of the Pinctada maxima N66 organic matrix protein. Aquaculture Research, 40 (9). pp. 1054-1063.
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There has been wide speculation about the functional importance, and potential contribution to the varying nacre characteristics of pearl oysters, of variation in the length of an asparagine-glycine-rich repeat domain (the NG region) within the key organic matrix proteins nacrein and N66. This speculation has centred on large inter-species differences in the length of the NG protein domain. We report for the first time the presence of significant intra-specific (allelic) polymorphismin both the length and the amino acid sequence of the NG repeat domain of N66 within both wild and hatchery populations of Pinctada maxima. We also detected the presence of a second putative N66-like locus within the P. maxima genome. Confirmation of gene expression of the various alleles at the two loci identified in P. maxima is now required, together with experimental testing of functional differences in the carbonic anhydrase activity of the alleles identified.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||aquaculture; OIRS; AAC; nacrein; N66; Pinctada maxima; biomineralization; nacre; pearl|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 90%|
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 10%
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830104 Aquaculture Oysters @ 100%|
|Deposited On:||19 Apr 2010 08:46|
|Last Modified:||15 May 2013 01:09|
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|Citation Counts with External Providers:||Web of Science: 3|
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