Relative uptake of technetium 99m stannous colloid by neutrophils and monocytes is altered by gram-negative infection
Ramsay, Stuart C,, Maggs, Jacqueline A., Ketheesan, Natkunam, Norton, Robert, and LaBrooy, Justin (2005) Relative uptake of technetium 99m stannous colloid by neutrophils and monocytes is altered by gram-negative infection. Nuclear Medicine and Biology, 32 (1). pp. 101-107.
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Gram-negative infection alters phagocytic cell function; hence, it could affect phagocytic uptake of inorganic colloids by these cells. Neutrophil and monocyte uptake of technetium 99m stannous colloid (99mTc SnC) in whole blood was measured in 10 patients with gram-negative infection (Burkholderia pseudomallei) and 7 controls. Mean uptake per individual neutrophil was reduced in infection. Uptake per monocyte was not significantly different. Blood from six normal individuals was incubated with lysed B. pseudomallei and colloid, which showed reduced neutrophil uptake, but increased monocyte uptake. These results indicate that uptake of 99mTc SnC stannous colloid can be used to measure alteration in phagocytic cell function. They suggest that infection with B. pseudomallei is associated with reduced phagocytosis by individual neutrophils, possibly through toxic effects of bacterial products. This could have immunopathogenic consequences for this gram-negative infection and may explain why it responds to granulocyte colony-stimulating factor.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||phagocytosis; radiocolloid; gram-negative sepsis; melioidosis; Burkholderia pseudomallei|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 10%|
11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 70%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 20%
|SEO Codes:||92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%|
|Deposited On:||16 Apr 2010 09:09|
|Last Modified:||22 May 2013 01:10|
Last 12 Months: 0
|Citation Counts with External Providers:||Web of Science: 4|
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